Mass cytometry provides a high-resolution proteomic profile at the single cell level. If you have no flow cytometry experience, don’t worry, we’re here to guide ou and make your projects a success. It allows for more parameters to be measured than current flow cytometry techniques.If you’re familiar with flow cytometry techniques and analysis you’ll definitely have a head-start in mass cytometry - the approaches and techniques are very similar. Mass cytometry (and the Hleios in particular) is primarily an extension of, or complement to, traditional fluorescence-based flow cytometry analysis. Unparalleled high dimensional analysis of cells The Fluidigm Helios is a 3rd generation CyToF instrument with top of the range sensitivity $6 - ask for list of currently stocked antibodies

Here, you will also find guides and resources to help you get up and running with your projects. We offer a full service and can assist you with all aspects of your mass cytometry applications: The OHRI Mass Cytometry Service (part of the Proteomics Core Facility) was established in 2019 and offers a state-of-the-art instrument and a mass cytometry specialist to help you establish and complete your projects.

Identify cells with phenotypic markers and then examine which signaling pathways are active.

Combine phenotyping with interrogation of signaling pathways.

More markers = better insight into which specific cells comprise heterogeneous populations.

Deeper phenotyping of heterogeneous populations.

The increased number of probes allows users to gain more insight: This means we can increase the number of parameters used - mass cytometry experiments typically use 30-50 probes.

Rare-earth metal probes have a fraction of the spillover of fluorescent probes.

By changing the traditional fluorophore probes in flow cytometry to rare-earth metals we overcome many of the problems associated with flow cytometry: Mass cytometry or Cytometry by Time of Flight (CyToF) is a modern technique that leverages the power of traditional cytometry techniques (staining millions of cells in suspension with labelled probes) with the sensitivity and specificity of mass spectrometry.

Hierarchical Stochastic Neighbour Embedding.

Uniform Manifold Approximation and Projection.

t-Distributed Stochastic Neighbour Embedding.

Assigning antibodies to labels (metals or fluorophores).

Which channels are available to assign antibodies to.

Identify and Source - Reagent Acquisition.

Planning out your mass cytometry project.Unparalleled high dimensional analysis of cells.

Boolean gating in flowjo 10